Optimization of multiplex polymerase chain reaction method for the amplification of microsatellite marker of Dishevelled 3 gene in human meningiomas

Skoko, Josip (2015) Optimization of multiplex polymerase chain reaction method for the amplification of microsatellite marker of Dishevelled 3 gene in human meningiomas. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Meningiomas are frequent benign tumours derived from the arachnoid cells. It has been well documented that wnt genes and other components of wnt signaling are critical for mammalian embryogenesis. Wnt signaling is essential in development because it acts as a regulator of the embryonic cell patterning, proliferation, differentiation, cell survival and apoptosis. The Wnt proteins bind to receptors of the Frizzled family on the cell surface. Through several cytoplasmic relay components, the first one being protein Dishevelled, the pathway is activated and the beta-catenin destruction complex is inhibited. The result is accumulation of beta-catenin in cytoplasm, which then enters the nucleus to activate transcription of Wnt target genes and tumorigenesis begins. Multiplex polymerase chain reaction is method used to analyze genetic changes and genomic instability of gene DVL3. We have achieved optimization of the method and we have specifically amplified the microsatellite marker of Dishevelled 3 gene along with control markers SHGC-58373 i APEX1. Further research is needed to establish the role of DVL3 gene and Wnt signaling in tumorigenesis of menigioma. The marker proved useful in revealing 2 MSI (9,52%) and 5 amplifications (23,81%) of DVL3 gene in our meningioma sample.

Item Type: Thesis (Diploma thesis)
Keywords: tumor, signaling pathway, WNT, optimization
Supervisor: Pećina-Šlaus, Nives
Co-supervisor: Oršolić, Nada
Date: 2015
Number of Pages: 45
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 17 Apr 2015 09:02
Last Modified: 17 Apr 2015 09:02
URI: http://digre.pmf.unizg.hr/id/eprint/3828

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