Effect of propolis ethanol extract on viabillity of P19 neurons in the presence of toxic concentrations of copper ions

Ukić, Petra (2015) Effect of propolis ethanol extract on viabillity of P19 neurons in the presence of toxic concentrations of copper ions. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Oxidative stress is one of the key factors involved in neuronal injury. Excess of copper ions can lead to the accumulation of toxic reactive oxygen species (ROS), especially hydroxyl radicals, via the Fenton chemistry, directly contributing to the development and progression of various neurodegenerative diseases. Propolis, a complex mixture rich in polyphenols, exerts great antioxidative activity. Therefore, the aim of the present study was to investigate the effect of alcoholic extract of propolis on oxidative damage and cell death provoked by toxic levels of copper in the culture of P19 neurons. In a dose-dependent manner, exposure to excess copper for 24 hours reduced survival of P19 neurons, while toxic effects of alcoholic extract of propolis were observed only at very high concentrations. Unexpectedly, alcoholic extract of propolis further decreased survival of P19 neurons exposed to 1 mM copper sulfate (CuSO4). Neurotoxic action of propolis was induced by increased accumulation of ROS and more pronounced caspase-3/7 activity, as well as with changes in the expression of p53 and c-fos mRNA. The obtained results suggest a pro-oxidant and pro-apoptotic potential of alcoholic extract of propolis during neuronal oxidative injury induced by high concentrations of copper ions, and indicate that usage of alcoholic extract of propolis might be contraindicated in patients with copper-related neurodegenerative diseases.

Item Type: Thesis (Diploma thesis)
Keywords: oxidative stress, neurotoxicity, apoptosis, caspase-3/7, p53, c-fos
Supervisor: Jazvinšćak Jembrek, Maja
Co-supervisor: Oršolić, Nada
Date: 2015
Number of Pages: 55
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 17 Apr 2015 09:17
Last Modified: 17 Apr 2015 09:17
URI: http://digre.pmf.unizg.hr/id/eprint/3831

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