Molecular study of HLA-B*44 alleles and haplotypes in the Croatian population

Špoljarić, Nikolina (2015) Molecular study of HLA-B*44 alleles and haplotypes in the Croatian population. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

In this study we analyzed the HLA genes (HLA-A, -B, -C and -DRB1) among 1727 unrelated voluntary hematopoietic stem cells donors from the Croatian Bone Marrow Donor Registry (CBMDR), as well as HLA-B*44 haplotypes. All subjects were tested by polymerase chain reaction and primers specific for a particular allele or group of alleles (methods: PCR-SSP and PCR-SSO). The most common HLA genes were: HLA-A*02 (31.5%), B*35 (12.9%), C*07 (26.1%) and DRB1*11 (17.5%). Total of 316 patients were analyzed for alleles B*44, which is one of the most common HLA-B genes in CBMDR. The most common allele was B*44:02 (41.4%), followed by B*44:03 (25.7%), B*44:27 (18.5%), B*44:05 (14.1%), while B*44:06 was observed only in one patient. Haplotype analysis showed that the most common haplotypes among B*44 positive subjects were: HLA-A*02:01-B*44:02-C*05:01- DRB1*04:01, HLA-A*23:01-B*44:03-C*04:01-DRB1*07:01, HLA-A*02:01-B*44:05- C*02:02-DRB1*16:01 and HLA-A*02:01-B*44:27-C*07:04-DRB1*16:01. Results of this study are consistent with the results reported for other European populations, except for the haplotype HLA-A*02:01-B*44:27-C*07:04-DRB1*16:01, which was described for the first time in the present study.

Item Type: Thesis (Diploma thesis)
Keywords: HLA genes, B*44, HLA haplotypes, linkage disequilibrium, population research
Supervisor: Grubić, Zorana
Date: 2015
Number of Pages: 56
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 20 Apr 2015 11:23
Last Modified: 20 Apr 2015 11:23
URI: http://digre.pmf.unizg.hr/id/eprint/3837

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