Differential and Cytochemical Analysis of Blood Cells in Rusty Blenny (Parablennius sanguinolentus Pallas, 1814)

Jarak, Matea (2013) Differential and Cytochemical Analysis of Blood Cells in Rusty Blenny (Parablennius sanguinolentus Pallas, 1814). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

This thesis presents results attained from peripheral blood of the fish Parablennius sanguinolentus. It contains erythrocyte, thrombocyte, limphocyte, monocyte, neutrophile and basophile counts. The May-Grünwald Giemsa stain was used in morphological and quantitative blood cell analysis. Cytological characterization of all blood cell types used differential cytochemical stains. The alcian blue (AB) dye and the periodic acid Schiff method (PAS) stained glycoconjugates and polysacharides. The sudan black B (SBB) dye stained cell lipids. Activities of alpha-naphthyl acetate esterase (ANAE), alpha-naphthyl butyrate esterase (ANBE) and alkaline phosphatase (ALP) were proved using enzyme-cytochemical reactions. Leukocyte and erithrocyte count, percentage and cell morphology are similar to other fish species from order Perciformes. AB stained nuclei of all cells. PAS stained lymphocytes, monocytes, neutrophils and thrombocytes. SBB stained lymphocytes, neutrophils and monocytes. ANAE did not show reaction in any type of cell. ANBE stained lymphocytes, neutrophils and monocytes. APFB showed reactions in neutrophiles and monocytes.

Item Type: Thesis (Diploma thesis)
Keywords: Parablennius, hematology, blood cells, cytochemistry
Supervisor: Đikić, Domagoj
Date: 2013
Number of Pages: 53
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 20 Apr 2015 11:53
Last Modified: 27 Apr 2017 08:43
URI: http://digre.pmf.unizg.hr/id/eprint/3844

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