Role of Gata4/6 transcription factors in differentiation of human embryonic stem cells in vitro

Josipović, Pavle (2013) Role of Gata4/6 transcription factors in differentiation of human embryonic stem cells in vitro. Doctoral thesis, Faculty of Science > Department of Biology.

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Human embryonic stem cells (hESC) can serve as a model for studying the development of primitive extraembryonic endoderm. Spontaneous differentiation of inner cell mass cells is a result of growing hESC in a cell culture. GATA transcription factors are crucial elements in the development of primitive extraembryonal endoderm. In our study we have focused on the expression of transcription factors Gata4 and Gata6, the primitive endoderm marker Sox17, as well as the expression of pluripotency genes Oct4, Sox2 i Nanog. Real time PCR has showed high expression Gata4, Gata6 and Sox17 during the spontaneous differentiation in all differentiating systems. We have explored the impact of three diverse differentiation system - matrigel, murine embryonic fibroblasts and embryoid bodies - on the development of primitive endoderm and expression of above mentioned markers. The presence of GATA4 and GATA6 proteins in the nuclei of differentiated embryonic stem cells and their specific spatial distribution was demonstrated by immunostaining. In order to investigate the mechanisms of early embryonic differentiation we generated hESC transgenic lines (VprOrgGata6GFP, VprOrgGata6mCherry, VprOrgCD41aGFP). Genetically modified hESC can serve as a model of early embryogenesis in vitro.

Item Type: Thesis (Doctoral thesis)
Keywords: GATA4, GATA6, Primitive endoderm, hESC, spontaneous differentiation
Supervisor: Marijanović, Inga and Zaninović, Nikica
Date: 2013
Number of Pages: 86
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Feb 2014 12:09
Last Modified: 22 Sep 2014 11:05

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