Effect of salt stress on potato virus Y-resistant dihaploid lines of tobacco (Nicotiana tabacum l.)

Marček, Tihana (2013) Effect of salt stress on potato virus Y-resistant dihaploid lines of tobacco (Nicotiana tabacum l.). Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Salinity is an important abiotic factor that limits plant growth and development. F1 hybrid DH10 and tobacco (Nicotiana tabacum L.) dihaploid lines, obtained by diploidization of anther-derived haploids of the F1 hybrid DH10 were exposed to sodium chloride (100 i 200 mM) in in vitro conditions. The line 207B, which showed the best resistance to Potato virus Y (PVY) (ICTV) in earlier research, was also tolerant to salinity, which was estimated on the basis of maximum growth index and the least pronounced symptoms. Measuring biochemical changes in studied dihaploids, different mechanisms of response to increased salinity were observed. In dihaploids 207B and 239K there was no significant accumulation of sodium. The increased proline accumulation was noticed in the shoots of lines 207B and 238C as well as in F1 hybrid DH10. All dihaploid lines and F1 hybrid DH10 showed an enhanced expression of SOD (Cu/Zn SOD) in response to salinity. In line 239K increased catalase activity had a significant role in detoxification of reactive oxygen species (ROS). Expression of dehydrins and heat - shock proteins (HSP70) as well as RuBisCO did not prove to be sufficiently specific indicators of F1 hybrid DH10 and dihaploid lines response to salt stres.

Item Type: Thesis (Doctoral thesis)
Keywords: Salt stress, tobacco dihaploid lines, proline, stress proteins, Potato Y virus (PVY)
Supervisor: Ćurković Perica, Mirna and Vidaković-Cifrek, Željka
Date: 2013
Number of Pages: 159
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Feb 2014 12:28
Last Modified: 22 Sep 2014 12:27
URI: http://digre.pmf.unizg.hr/id/eprint/392

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