Effects of bee venom and melittin on cell response of tumor and non-tumor cells in vitro

Gajski, Goran (2012) Effects of bee venom and melittin on cell response of tumor and non-tumor cells in vitro . Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Possible anticancer ability of bee venom and its major component melittin towards different types of tumour cells and their drug resistant sublines as well as their impact on normal non-tumour cells was investigated. After the treatment with whole bee venom or melittin all the cell lines tested displayed dose dependent cytotoxicity. In addition, treatment with bee venom and melittin caused significant morphological changes and induced dominantly necrotic type of cell death. Following a combined treatment with chemotherapeutic drug cisplatin, bee venom exhibited additive effect what could be useful from the point of minimizing cisplatin concentration during chemotherapy. Bee venom and melittin were toxic to normal cells as well; causing lover cell viability, morphological cell alterations and DNA damage. Depletion of glutathione and increment in malondialdehid concentration parallel with oxidative DNA damage and induction of phospholipase C activity, suggest that both bee venom and melittin act through lipid peroxidation and that oxidative stress is at least partly involved in their mode of action. Although toxic to all cell types tested, toxicity of bee venom and melittin was cell type dependent making tumour cells more sensitive to both, as compared to the non-tumour cells. Depending on the origin, drug resistant cells could be more sensitive to bee venom and melittin than parental cells. Although toxic to all the cell lines tested, our results speak in favour of application of bee venom and melittin in anticancer therapy.

Item Type: Thesis (Doctoral thesis)
Keywords: bee venom, melittin, cisplatin, cytogenotoxicity, oxidative stress, tumour cells, non-tumour cells
Supervisor: Garaj Vrhovac, Vera
Date: 2012
Number of Pages: 173
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Feb 2014 12:42
Last Modified: 16 Feb 2016 13:55
URI: http://digre.pmf.unizg.hr/id/eprint/393

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