HLA allele polymorphisms among patients in hematopoietic stem cell transplantation program

Šamija, Zvonimir (2015) HLA allele polymorphisms among patients in hematopoietic stem cell transplantation program. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The aim of the present study was to analyze the distribution of HLA alleles and haplotypes among patients (N=150) in hematopoietic stem cell transplantation program (HSCT) in Croatia, in order to determine the probability of finding an HLA matched unrelated donor. The distribution of HLA-A, -B, -C, -DRB1, -DQB1 alleles and HLA-A-B-DRB1/HLA-A-B-C-DRB1-DQB1 haplotypes was investigated. HLA alleles were tested by Polymerase Chain Reaction with specific primers or probes (PCR-SSP and PCR-SSO). HLA haplotypes were determined by segregation analysis. Comparison of patients included in this study and healthy controls regarding the HLA allele distribution did not reveal any statistically significant difference. The most common alleles detected in our patient group were: HLA-A*02:01 (27.7%), B*51:01 (13.0%), C*07:01 (20.3%), DRB1*03:01 (15.3%) and DQB1*03:01 (23.3%). Out of 227 different detected HLA-A-B-DRB1 haplotypes, 197 were observed only once and the most frequent one was HLA-A*01:01-B*08:01-DRB1*03:01 (7.3%). Five loci haplotype analysis confirmed the highest frequency of HLA-A*01:01-B*08:01-C*07:01-DRB1*03:01-DQB1*02:01 haplotype, which is in concordance with the previous studies. Unusual HLA-B-C combinations detected in 15 patients together with unusual HLA-DRB1-DQB1 combinations detected in four patients and the presence of rare alleles (A*29:01, A*80:01, C*07:18, DRB1*04:15, DRB1*11:15, DRB1*11:28) were shown to be limiting factors in finding an HLA matched unrelated donor.

Item Type: Thesis (Diploma thesis)
Keywords: HLA, hematopoietic stem cell transplatation, unrelated donor
Supervisor: Grubić, Zorana
Date: 2015
Number of Pages: 43
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 12 May 2015 08:45
Last Modified: 12 May 2015 08:45
URI: http://digre.pmf.unizg.hr/id/eprint/3961

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