Diversity of mitochondrial DNA in wildcat (Felis silvestris) in Croatia

Jagić, Mateja (2014) Diversity of mitochondrial DNA in wildcat (Felis silvestris) in Croatia. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Wildcat (Felis silvestris) is autochthonous and strictly protected species in Croatia; however there is almost none scientific data on Croatian wildcat population. The goal of this research was to investigate diversity of mitochondrial DNA control region of wildcat from Croatia and to compare identified haplotypes with haplotypes of domestic cat (Felis catus). A total of 41 samples of wildcats and 38 domestic cat samples were included in the research. I analysed 814 base pairs long control region sequences and in wildcat samples 22 polymorphic sites were identified yielding 11 different haplotypes. Haplotype diversity of analysed sequences was 0,857 +/- 0,036, while nucleotide diversity was 0,017 +/- 0,009. Among 38 domestic cats samples 21 polymorphic sites were identified yielding 11 haplotypes. Haplotype diversity of analysed sequences of domestic cats was 0,758 +/- 0,067, while nucleotide diversity was 0,011 +/- 0,006. There were no matches between wildcat and domestic cat haplotypes. Based on these results I conclude that mitochondrial DNA control region diversity of wildcat from Croatia is fairly high.

Item Type: Thesis (Diploma thesis)
Keywords: wildcat, domestic cat, genetic diversity, haplotype, polimorfic site
Supervisor: Galov, Ana
Date: 2014
Number of Pages: 34
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 12 May 2015 09:26
Last Modified: 12 May 2015 09:26
URI: http://digre.pmf.unizg.hr/id/eprint/3965

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