Effects of FGF and HMGB1 proteins on Cultured Neural Stem Cells' Survival and Proliferation

Bogut, Ana (2014) Effects of FGF and HMGB1 proteins on Cultured Neural Stem Cells' Survival and Proliferation. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The aim of this study was to describe the effects of FGF and HMGB1 molecules on cultured neural stem cells (NSCs). Using magnetofection, NSCs were transfected with plasmides containing the FGFR1 receptor. Along with NSCs, embryonal kidney stem cells (HEK293T) were also used. The results show that FGF positively affects the survival of HEK293T cells, which were transfected with plasmides, as opposed to non-transfected cells. The HMGB1 molecule reduced the positive effect of FGF in cultured HEK293T cells. NSCs were already grown in a medium containing FGF. The ones transfected with the FGFR1-EGFP plasmide showed increased survival. Using twice as many plasmides as magnet particles resulted in a twofold increase in magnetofection success rate, compared to previous studies. At the same time, HMGB1 affected their survival negatively, just as in HEK293T cells.

Item Type: Thesis (Diploma thesis)
Keywords: neural stem cells, transfection, FGF, HMGB1
Supervisor: Mitrečić, Dinko
Co-supervisor: Marijanović, Inga
Date: 2014
Number of Pages: 55
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 13 May 2015 09:38
Last Modified: 13 May 2015 09:38
URI: http://digre.pmf.unizg.hr/id/eprint/3970

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