DNA barcoding as a method in taxonomical identification of plant and animal species

Ćosić, Jakob (2014) DNA barcoding as a method in taxonomical identification of plant and animal species. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

DNA barcoding is a newly proposed method of molecular taxonomy, which by the usage of short, universal sequences that are divergent enough in all organisms of interest, allows fast and precise identification of biological material. In this paper, I examined the fidelity of DNA barcoding as a taxonomic method for identification of plant and animal species. I also barcoded nine autochthonous Croatian plant species and chamois Rupicapra rupicapra L. (Bovidae), and conducted analysis of phylogenetic relations of chamois populations from Croatia based on collected barcode sequences. In the end, I considered the problems concerning the method, as well as suggestions for its improvement. Considered problems are mostly small differences between barcodes of different organisms, problematic barcoding of plants due to slow evolution of plastid genome and isolation of plant genes and problematic determination of animals due to specific evolution of animal mitochondrial genome.

Item Type: Thesis (Diploma thesis)
Keywords: molecular taxonomy, matK, rbcL, COI, chamois
Supervisor: Franjević, Damjan
Date: 2014
Number of Pages: 50
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 13 May 2015 10:09
Last Modified: 13 May 2015 10:09
URI: http://digre.pmf.unizg.hr/id/eprint/3972

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