Cell-death factors expression in neuroblastoma

Bogović, Marko (2013) Cell-death factors expression in neuroblastoma. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

The aim of this study was to investigate the correlation between the expression of cell death factors - Fas, FasL, perforin and HSP70 and biological characteristics of neuroblastoma. All children diagnosed with neuroblastoma operated at KBC Zagreb in the period since 1994. to 2013. were included and 42 of them met our criteria. On paraffin blocks excluded before the start of chemotherapy through immunohistochemical staining the expression of Fas and FasL molecules on neuroblastoma cells, the number and expression of perforin on cytotoxic T lymphocytes in the vicinity of neuroblastoma cells and expression of HSP70 in neuroblastoma cells was analyzed. Reduced Fas expression was found in metastatic tumor stages so it can be assumed that by lowering expression of Fas on its surface, tumor can avoid the local immune response and thus metastasize. The connection of HSP70 expression and stage neuroblastoma is also found. Higher stages have decreased expression of which is in contrast with the majority of tumors that have been studied, but on the other hand agrees with research on nephroblastoma, the second most common solid tumor in children. The most likely explanation for that, according to available data and considering pediatric tumors, is that the role of HSP70 in inhibition of apoptosis is not as important as participation in the differentiation of the tumor and its overexpression in neuroblastoma leads to the maturation of the tumor.

Item Type: Thesis (Doctoral thesis)
Keywords: neuroblastoma, Fas, FasL, perforin, HSP70, cytotoxic T cells
Supervisor: Luetić, Tomislav and Mrakovčić-Šutić, Ines
Date: 2013
Number of Pages: 91
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Feb 2014 13:47
Last Modified: 10 Feb 2014 13:47
URI: http://digre.pmf.unizg.hr/id/eprint/398

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