The role of p73 isoforms on DNA methylation in cancer cells

Tučkar, Neven (2012) The role of p73 isoforms on DNA methylation in cancer cells. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

The role of p73 in tumorigenesis is not fully understood. Using P1 promoter transactivating (TAp73) isoforms are generated and P2 promoter the isoforms without this region (ΔNp73). The most common change in tumors is increased expression of ΔNp73, which acts as transdominant inhibitor of TAp73 and p53. DNA methylation is a form of epigenetic regulation, which adds methyl groups to cytosine residues, regulating gene activity. Hypermethylation of CpG islands in promoter regions inhibits transcription of genes silencing its expression. Using inducible tet-on system, we examined the impact of increased expression of TAp73α and ΔNp73α on global DNA methylation. However statistically significant change was not found upon induced expression of p73 isoforms. The results were compared with gene expression data obtained after induction of TAp73α or ΔNp73α from literature. For genes with increased expression we found, although not statistically significant, reduction of methylated CpG sites in promoter regions, which confirms the fact that methylation could affect the regulation of gene activity.

Item Type: Thesis (Doctoral thesis)
Keywords: TP73, p73 isoforms, TAp73α, ΔNp73α, methylation
Supervisor: Slade, Neda
Date: 2012
Number of Pages: 75
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Feb 2014 13:59
Last Modified: 11 Sep 2017 09:01
URI: http://digre.pmf.unizg.hr/id/eprint/399

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