The development of methods for DNA microsatellites analysis and their applicationfor bovine identification

Projić, Petar (2013) The development of methods for DNA microsatellites analysis and their applicationfor bovine identification. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Tetra nucleotides STR systems are used to analyse the DNA of human origin. In the analyses of samples of animal origin dinucleotide microsatellites are most commonly used. For the study of bovine origin International Society for Animal Genetics (ISAG) has recommended 30 of the most common dinucleotide microsatellites that are present in different combinations in existing commercial kits. By using one of available tools (Tandem-Repeats Finder) for "in silico mining" search of microsatellite sequences from a database of DNA sequences (UniGene NCBI) for cattle, a new highly polymorphic microsatellites were chosen - up till now not applied in the procedures of establishing the bovine identity. They were tested on a sample of 110 cattle and compared with microsatellites included in the existing commercial kit of Applied Biosystems. The large number of the existing, recommended microsatellites was also examined. Then the statistically most significant combination was chosen– newly developed multiplex STR system -12 STR loci and the allelic ladder for selected combination has been developed and integrated. The acquired results will enable the aplication of markers the use of which will increase the value and accuracy of future DNA analysis in cattle. It will also bring them closer to the DNA analysis of human origin.

Item Type: Thesis (Doctoral thesis)
Keywords: in silico mining, cattle (Bos Taurus), microsatellite, new markers, population studies
Supervisor: Primorac, Dragan
Date: 2013
Number of Pages: 116
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Feb 2014 14:05
Last Modified: 22 Sep 2014 19:25
URI: http://digre.pmf.unizg.hr/id/eprint/400

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