Effect of modulated radiofrequency radiation on oxidation-reduction equilibrium of animal cells

Marjanović, Ana Marija (2015) Effect of modulated radiofrequency radiation on oxidation-reduction equilibrium of animal cells. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Aim of the study was to assess the effect of radiofrequency (RF) radiation at nonthermal level on cellular oxidation-reduction balance and development of oxidative stress. Fibroblast V79 and neuroblastoma SH-SY5Y cell lines were exposed to modulated RF radiation (1800 MHz, 30 V/m, 1.6 W/kg) for 10, 30 and 60 minutes. Electromagnetic field was generated within gigahertz transversal electromagnetic mode cell (GTEM) equipped by signal generator, amplifier and modulator. Viability, concentration of total and oxidised proteins, level of reactive oxygen species (ROS) and concentration of glutathione were determined by spectroscopic methods. In V79 cells increased ROS level was balanced by enhanced antioxidant activity without damage to macromolecular structures. A significant ROS increase and decrease of antioxidant capacity of SH-SY5Y cells was connected to radiation exposure. Significant oxidative damage to proteins and lipids was observed after 60-minute irradiation. According to oxidation-reduction status, SH-SY5Y cells were more sensitive to RF radiation compared to V79 cells. Results showed adverse effect of nonthermal RF radiation at molecular level disrupting cellular oxidation-reduction balance.

Item Type: Thesis (Doctoral thesis)
Keywords: Global System for Mobile Communication (GSM), nonthermal effects, reactive oxygen species (ROS), oxidative damage, antioxidative defence
Supervisor: Trošić, Ivančica
Date: 2015
Number of Pages: 105
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 27 May 2015 10:53
Last Modified: 27 May 2015 10:53
URI: http://digre.pmf.unizg.hr/id/eprint/4017

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