Cytokines in human immunodeficiency virus type 1 inefction

Gorenec, Lana (2015) Cytokines in human immunodeficiency virus type 1 inefction. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

The aim of this study was to compare cytokine expression on both gene and protein levels in acute and chronic phase of HIV type 1 infection and to analyze the effect of long-term suppression of viral replication in period longer than 2 years on cytokine immunity. To analyze cytokine expression on protein level in acute and chronic phase of HIV type 1 infection 34 patients were enrolled and the effect of long-term suppression of viral replication was determined in 80 HIV-positive patients. Using PCR array technology, expression of 84 cytokine genes was measured in 3 patients in acute and 3 patients in chronic phase of HIV type 1 infection. Bead-based cytometry was used to quantify levels of Th1/Th2/Th9/Th17/Th22 cytokines. The results showed statistically significant increase of 13 cytokine gene expression (cd40lg, csf2, ifna5, il12b, il1b, il20, lta, osm, spp1, tgfa, tnfsf 11, 14 and 8) and down regulation of the il12a expression in chronic HIV type 1 infection. Concentrations of IL-10, IL-4 and TNF-α were increased in the acute HIV type 1 infection when compared to control group. During chronic HIV type 1 infection there was an increase of IL-10, TNF-α, IL-2, IL-6, IL-13 and IL-22 levels when compared to control group. Comparison of cytokine expression between two stages of infection showed significant decrease in IL-9 concentration. Level of IL-17 was lower in therapy-naive patients with a suppression of viral replication. Expression of activation markers CD38 and HLA-DR was lower in a chronic stage of infection compared to acute infection as well as in samples with suppression of viral replication compared to samples before therapy initiation. This study showed change in cytokine profile in both gene and protein expression in different stages of HIV-infection.

Item Type: Thesis (Doctoral thesis)
Keywords: HIV type 1, biological response modifiers, immune activation
Supervisor: Židovec Lepej, Snježana
Date: 2015
Number of Pages: 131
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 27 May 2015 11:11
Last Modified: 27 May 2015 11:11
URI: http://digre.pmf.unizg.hr/id/eprint/4021

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