DNA repair capacity in natural mussel Mytilus galloprovincialis population

Smodlaka, Mirta (2015) DNA repair capacity in natural mussel Mytilus galloprovincialis population. Doctoral thesis, Faculty of Science > Department of Geology.

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Abstract

DNA repair is a key mechanism for the genome integrity maintenance and defense against the consequences of genotoxic pollutants. The DNA repair capacity in marine bioindicator species is an important biomarker for the marine environment monitoring that demand a simple and economic method. Following optimization of the 3D method a new protocol was established that allows a fluorimetric measurement of the DNA repair capacity in the digestive gland and gills of mussel Mytilus galloprovincialis was tested. The DNA repair capacity in the digestive gland was higher than in the gills suggesting that DNA repair capacity is tissue specific. The correlation between the DNA repair capacities of investigated tissues indicates a coordinated response of the organism to genotoxic pressure. DNA repair capacity in digestive gland distinguished population living under different anthropogenic pressure as well as correlated with the DNA integrity in haemocytes as indicators of environmental genotoxic pressure. Introduction of the 3D method measurement of DNA repair capacity into the ecotoxicological studies could improve the assessment of the genotoxic risks in the marine environment.

Item Type: Thesis (Doctoral thesis)
Keywords: DNA repair, DNA integrity, genotoxicity, mussel, 3D assay, flow cytometry, Adriatic Sea
Supervisor: Fafanđel, Maja
Date: 2015
Number of Pages: 101
Subjects: NATURAL SCIENCES > Geology > Geology and Paleontology
Divisions: Faculty of Science > Department of Geology
Depositing User: Danica Vujičić
Date Deposited: 01 Jul 2015 12:25
Last Modified: 01 Jul 2015 12:25
URI: http://digre.pmf.unizg.hr/id/eprint/4063

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