The role of “notch” signaling in differentiation of hematopoietic cells

Paradžik, Mladen (2015) The role of “notch” signaling in differentiation of hematopoietic cells. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Differentiation of hematopoietic stem cells into functional mature cells is controlled by a wide array of transcriptional regulators. Ikaros family transcription factors and the Notch signaling pathway are important parts of these processes. It was shown that Ikaros members are essential for the lymphoid development and aberrant changes in their expression could lead to lymphoma expansion in mice. Notch signaling also has a key role in directing the hematopoietic cell fate and its role is mainly confirmed in T lymphocyte development. However, the importance of the Notch signaling in the development of B lymphocytes and myeolid lineage remains poorly understood. The aim of this study is to define the correlation of Ikaros and Notch families in the differentiation of the lymphoid and myeloid lineages, using molecular techniques such as single cell multiplex PCR, real time PCR, Western blotting and flow cytometry. It was confirmed that Ikaros family members interact with the members of Notch signaling pathway. Furthermore, Notch plays a key role in myeloid differentiation and it is important factor in the regulation of the apoptosis in B cell chronic lymphocytic leukemia.

Item Type: Thesis (Doctoral thesis)
Keywords: Notch, Ikaros family, differentiation, hematopoesis, leukemia
Supervisor: Antica, Mariastefania
Date: 2015
Number of Pages: 112
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 06 Jul 2015 09:01
Last Modified: 06 Jul 2015 09:01
URI: http://digre.pmf.unizg.hr/id/eprint/4089

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