Nicotiana glauca Graham – potential cadmium phytoremediator

Majsec, Kristina (2015) Nicotiana glauca Graham – potential cadmium phytoremediator. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Heavy metal pollution is a result of human actions, and their increasing presence has serious consequences for the environment, as well as for human health. Cadmium (Cd) is one of the most toxic metals. Because of its mobility, accumulation in the food chain and wide application in industry, Cd is a threat for human health and the entire environment. Many plant species have developed tolerance for heavy metals in the environment, by utilizing two distinct strategies: accumulation strategy and exclusion strategy. Understanding of accumulation mechanisms is especially important in agriculturally important crops in order to prevent heavy metals from entering the food chain and for possible use of plants for removal of metals from soils through the process of phytoremediation. The goal of this thesis was to compare mechanisms of tolerance to Cd in potential hyperaccumulator Nicotiana glauca Graham and moderately tolerant N. tabacum L. Results have shown that N. glauca is a Cd hyperaccumulator and a potential phytoremediator. In N. glauca Cd accumulation and tolerance is enabled by constitutively high expression of genes involved in heavy metal uptake and transport (ZIP, PDR3, HMA-A), Fe uptake and homeostasis (CIT1, FER), antioxidative defense (GST) and chelator synthesis (NAS). After Cd treatment in N. tabacum leaves there was a response similar to Fe-deficiency (induction of NRAMP1 expression) and drastic inhibition of ZIP transporter expression in the roots, in order to prevent further metals from entering the plant. In N. glauca Cd caused increased expression of gene involved in metal uptake and metal translocation to the leaves (HMA-A) which is an important trait of hyperaccumulators and potential phytoremediators. Obtained results show that gene HMA-A is a potential target for constructing transgenic N. glauca that could be used for Cd phytoremediation.

Item Type: Thesis (Doctoral thesis)
Keywords: Nicotiana glauca, hyperaccumulation, cadmium, heavy metal tolerance, Fe deficiency, phytoremediation
Supervisor: Pavlica, Mirjana
Date: 2015
Number of Pages: 102
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 06 Jul 2015 10:08
Last Modified: 06 Jul 2015 10:08
URI: http://digre.pmf.unizg.hr/id/eprint/4094

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