Characteristics of immune cells of weaned pigs treated with isoflurane and nitrous oxide

Lerotić, Ivan (2015) Characteristics of immune cells of weaned pigs treated with isoflurane and nitrous oxide. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

In this doctoral thesis pig is taken as a globally recognized model in biomedical research in order to identify potential adverse effects of anesthetics isoflurane and nitrous oxide permanent gas on cellular immunity after their multi-day repeated administration. This prospective, randomized, controlled trial involved 28 piglets in good general condition. Tested animals, depending on the treatment with isoflurane alone or in combination with the nitrogen oxide, were divided into groups. Blood samples for determination of hematologic, biochemical and immune parameters were taken at days 0, 4, 8, 16 and 24 of study. At the end of the study samples were taken from bone marrow, spleen, lung and lymph node and underwent histopathological examination. The results of these studies suggest that isoflurane and its combination with nitrogen oxide in pigs anesthetized for 24 days, have immunosuppressive effect or reduce stakes cell populations cellular immunity pigs (CD4+, CD8+, CD21+ and CD45+). However, in anesthetized piglets with nitrogen oxide and oxygen without addition of isoflurane for 24 days, at the end of the study was not recorded their harmful effect on the cellular immune system of animals. The results confirmed the initial hypothesis. The resulting immune indicators are a scientifically based recommendation that isoflurane and its combination with nitrogen oxide for the purpose of chronic anesthesia on a model of domestic pigs should be used with caution, especially if there is already a compromised immunity.

Item Type: Thesis (Doctoral thesis)
Keywords: immune cells, isoflurane, nitrous oxide, domestic pig
Supervisor: Vnuk, Dražen
Date: 2015
Number of Pages: 70
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 17 Jul 2015 11:52
Last Modified: 17 Jul 2015 11:52
URI: http://digre.pmf.unizg.hr/id/eprint/4142

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