Total phenolic and flavonoid contents of in vitro cultured Stevia rebaudiana (Bert)

Glogoški, Marko (2013) Total phenolic and flavonoid contents of in vitro cultured Stevia rebaudiana (Bert). Diploma thesis, Faculty of Science > Department of Biology.

[img] PDF
Restricted to Registered users only
Language: Croatian

Download (1MB) | Request a copy

Abstract

Stevia rebaudiana Bertoni is a sweet flavoured perennial plant from the family Asteraceae. It originated in Paraguay where it is used as a sweetener for more than 1500 years. The main source of sweetness are steviosides, which are found in leaves. Dry leaf extracts also contain flavonoids, phenols and many other compounds. The aims of this research were to determine the influence of the nutrient composition and growth hormones on seed germination and multiplication, as well as on the total content of phenols and flavonoids in different plant tissues of species S. rebaudiana. Following sterilizazion, the seeds were germinated on full or half-strength Murashige and Skoog (MS) media supplemented with growth regulators gibberellic acid (GA3) alone or in combination with 6-benzyl aminopurine (BA) in different concentrations. Nodal explants were transplanted on multiplication media and then rooted. Best seed germination is achieved by combination of GA3 and BA and best induction of growth by GA3. The highest content of phenols and flavonoids was achieved in callus tissue using combination of ndole-3-acetic acid (IAA) and BA, while the lowest was detected in roots. Higher concentration of IAA stimulates phenol storage in callus, and inhibits in leaf, while higher concentration of indole-3-butyric acid (IBA) does the opposite. Higher concentration of IAA inhibits flavonoids storage in leaf, while higher concentration of IBA hormone stimulates storage of flavonoids in both leaf and callus tissue.

Item Type: Thesis (Diploma thesis)
Keywords: stevia, antioxidants, multiplication, seed germination, rooting, axillary shoot induction
Supervisor: Radić Brkanac, Sandra
Date: 2013
Number of Pages: 50
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 12 Feb 2014 12:59
Last Modified: 23 Sep 2014 10:42
URI: http://digre.pmf.unizg.hr/id/eprint/417

Actions (login required)

View Item View Item