Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores

Kajtez, Janko and Solomatina, Anastasia and Novak, Maja and Polak, Bruno and Vukušić, Bruno and Rüdiger, Jonas and Cojoc, Gheorghe and Milas, Ana and Šumanovac Šestak, Ivana and Risteski, Patrik and Tavano, Federica and Klemm, Anna H. and Roscioli, Emanuele and Welburn, Julie and Cimini, Daniela and Glunčić, Matko and Pavin, Nenad and Tolić, Iva Marija (2016) Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores. Nature Communications, 7. Article number 10298. ISSN 2041-1723

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Abstract

During metaphase, forces on kinetochores are exerted by k-fibres, bundles of microtubules that end at the kinetochore. Interestingly, non-kinetochore microtubules have been observed between sister kinetochores, but their function is unknown. Here we show by laser-cutting of a k-fibre in HeLa and PtK1 cells that a bundle of non-kinetochore microtubules, which we term ‘bridging fibre’, bridges sister k-fibres and balances the interkinetochore tension. We found PRC1 and EB3 in the bridging fibre, suggesting that it consists of antiparallel dynamic microtubules. By using a theoretical model that includes a bridging fibre, we show that the forces at the pole and at the kinetochore depend on the bridging fibre thickness. Moreover, our theory and experiments show larger relaxation of the interkinetochore distance for cuts closer to kinetochores. We conclude that the bridging fibre, by linking sister k-fibres, withstands the tension between sister kinetochores and enables the spindle to obtain a curved shape.

Item Type: Article
Keywords: biological sciences, cell biology
Date: 5 January 2016
Subjects: NATURAL SCIENCES > Biology
Additional Information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
Divisions: Faculty of Science > Department of Physics
Publisher: Nature Publishing Group
Depositing User: Gordana Stubičan Ladešić
Date Deposited: 19 Jan 2016 14:23
Last Modified: 29 Nov 2017 17:18
URI: http://digre.pmf.unizg.hr/id/eprint/4365

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