Development of molecular methods for identification of psychoactive fungi and their implementation in forensic

Horjan, Ivana (2016) Development of molecular methods for identification of psychoactive fungi and their implementation in forensic. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Most of psychoactive fungi contain psilocybin, psilocin, baeocystin or nor- baeocystin, and due to effects of psilocin, which are similar to LSD, they are illegal. Proving active substances from forensic samples through toxicological methods is not possible from spore print or from mycelia. Main goal of this thesis was to emphasize the potential of using molecular and morphological methods in forensic analysis of psychoactive fungi. Morphological analysis of 63 forensic samples resulted with 5 successfully identified species with Psilocybe cubensis as the most common. Through sequencing the ITS region and the part of LSU gene of forensic samples, morphological analysis results were confirmed and sixth species was successfully identified from spore print. Both methods can be implemented in the routine forensic workflow for fungi. Potential of the researched model for preliminary identification of psychoactive fungi by using specific TaqMan®MGB probes in RT-PCR reaction, was not confirmed. LC-MS analysis established the psychoactive status of species Stropharia semiglobata and Galerina badipes.

Item Type: Thesis (Doctoral thesis)
Keywords: sychoactive fungi, morphology, DNA sequencing, probes TaqMan®MGB, LC-MS analysis
Supervisor: Besendorfer, Višnja
Co-supervisor: Tkalčec, Zdenko
Date: 2016
Number of Pages: 131
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 04 Mar 2016 13:44
Last Modified: 04 Mar 2016 13:44
URI: http://digre.pmf.unizg.hr/id/eprint/4546

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