Polymorphism of interleukin 28B gene in human immunodeficiency virus infected individuals

Pehar, Sanja (2015) Polymorphism of interleukin 28B gene in human immunodeficiency virus infected individuals. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Infection with the human immunodeficiency virus (HIV) is associated with progressive deficiency of the immune system. CD4+ T-lymphocytes are the primary targets of HIV which leads to depletion of this cell population in HIV-infected individuals which serves as a prognostic marker for HIV disease progression. Interferons λ inhibt the replication of different viruses. Single-nucleotide polymorphism (SNP) of interleukin 28B (IL-28B, one of λ interferons) affects the concentration of that interleukin in the organism. C homozygotes have higher concentrations of that interleukin and therefore slower disease progression. The aim of this study is to determine the corellation between IL-28B SNP and immunological status of HIV-infected individuals. I have divided the patients into three groups considering their CD4+ count and using a real-time PCR test determined their IL-28B genotype. First group consisted of patients who had > 500 CD4+ lymphocytes / μl at entrance to care and at least for one year after. In the second group were patients who had < 200 CD4+ lymphocytes / μl but then thier CD4+ count rose to > 500 lymphocytes / μl and stayed as high for at least a year. Third group had patients who entered clinical care with < 200 CD4+ lymphocytes / μl at and remained so for at least a year. C homozygotes are most frequent in entire study group (50,5 %) as well as in the first and third group of patients. Heterozygotes were present in 36,7 % in the entire study group and were the most frequent in second group of patients. T homozygotes were the least frequent in an entire study population and all three groups. There were no statistically significant differences in genotype distribution between groups so this study showed that there is no corelation between disease progression and IL-28B genotype in HIV-infected individuals.

Item Type: Thesis (Diploma thesis)
Keywords: HIV, CD4+ T-cells, interferon, IL-28B
Supervisor: Židovec Lepej, Snježana
Co-supervisor: Marijanović, Inga
Date: 2015
Number of Pages: 38
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 04 Mar 2016 14:03
Last Modified: 04 Mar 2016 14:03
URI: http://digre.pmf.unizg.hr/id/eprint/4549

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