Indicators of oxidative stress in tobacco (Nicotiana tabacum L.) exposed to thallium (I) acetate

Prigorec, Tanja (2015) Indicators of oxidative stress in tobacco (Nicotiana tabacum L.) exposed to thallium (I) acetate. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Oxidative stress in plants occurs as a secondary reaction to heavy-metals stress. In this work I analysed the change of protein content, hydrogen peroxide, malondialdehyde, carbonyls as well as activity of ascorbate peroxidase, pyrogallol peroxidase and catalase as indicators of oxidative stress in the shoots of tobacco (Nicotiana tabacum L.) exposed to thallium (I) acetate. Plants were treated with 2, 4, 20 and 40 μM Tl-acetate, 40 μM Tl-acetate and 40 μM K-acetate (combined treatment) and 40 μM K-acetate which served as an additional control of the acetate. Samples of tobacco shoots were analyzed after 3 days. The protein content, as well as hydrogen peroxide and carbonyl content, increased in relation to the control, while MDA content did not change. Ascorbate and pyrogallol peroxidase activity and catalase activity increased significantly in plants exposed to 2 μM and 4 μM Tl-acetate. The research results show that thallium causes oxidative stress in tobacco shoots, but also the efficiency of antioxidative mechanisms of tobacco at different concentrations of that toxic metal used in this work.

Item Type: Thesis (Diploma thesis)
Keywords: tobacco, oxidative stress, thallium (I) acetate
Supervisor: Pevalek-Kozlina, Branka
Co-supervisor: Babić, Marija
Date: 2015
Number of Pages: 34
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 04 Mar 2016 14:18
Last Modified: 04 Mar 2016 14:18
URI: http://digre.pmf.unizg.hr/id/eprint/4551

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