Determination of species and hybrids of water frogs (genus Pelophylax) from Skadar Lake, Montenegro using molecular markers

Vucić, Matej (2015) Determination of species and hybrids of water frogs (genus Pelophylax) from Skadar Lake, Montenegro using molecular markers. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The study of water frogs from Skadar Lake (Montenegro) is conducted in order to clarify the number of species and hybrids of this interesting group of amphibians in this area. Water frogs (genus: Pelophylax) is an extremely complex group of animals with 25 described species and 3 hybrid complex of which Pelophylax kl. esculentus is the best studied one. Previous studies of water frogs on Lake Skadar based on morphological characteristics and complex molecular methods such as cytogenetics and allozyme electrophoresis indicate the existence of at least two species and their potential hybrid. Visual and morphological methods of determination of water frogs are not completely reliable so the objectives of this thesis are: to determine which species of water frogs are present in the area of Skadar Lake using mitochondrial and nuclear markers and determine whether there is a hybridization. Molecular research is based on simple method, where the species is determined by the the length of the albumin intron region. Hybrids are determined accordingly. Additionally, ND3 mitochondrial region was analyzed in order to determine species with certainty, since there are large sets of data in the open access sequence databases such as GenBank.

Item Type: Thesis (Diploma thesis)
Keywords: Balkan Peninsula, Pelophylax kurtmuelleri, P. shqipericus, length variation of serum albumin gene intron-1, mitochondrial region ND3
Supervisor: Klobučar, Goran
Co-supervisor: Jelić, Mišel
Date: 2015
Number of Pages: 48
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 08 Mar 2016 12:13
Last Modified: 08 Mar 2016 12:13
URI: http://digre.pmf.unizg.hr/id/eprint/4554

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