Glycomic phenotyping of twins with lumbago

Vučenović, Dunja (2015) Glycomic phenotyping of twins with lumbago. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Lumbago is a common musculoskeletal condition in all ages. Owing to its clinical and social impact, a clear diagnosis of this syndrome is needed in order to define besides pain intensity also the interference of pain with daily and work activity. Currently, there are limited biomarkers (mostly imaging), but there is a need for novel and simpler detection methods. One of the major determinants of pain in persistent lumbago is localised inflammation in epidural space. As every inflammatory process is linked with changes on the inflamed region, so are altered N-glycan IgG compositions. In this study, using classical twin design conducted between twins from TwinsUK registry, I assessed whether twins reporting episodes of lumbago had detectable levels of altered IgG glycosylation. I analysed IgG N-glycome of 4416 twins having completed pain history questionnaires, whose glycan profile was determined and who underwent magnetic-resonance spine scan. With this data, using statistical methods for twin studies, I looked for association between glycan quantities and pain phenotypes. Additionally, I used methods applied in genomics and obtained modules of glycans that show different patterns of glycan quantities. With these modules I built prediction algorithms that are giving likelihood of person reporting episodes of lumbago.

Item Type: Thesis (Diploma thesis)
Keywords: lumbago, glycans, regression, mixed models, WGCNA, LASSO
Supervisor: Williams, Frances
Co-supervisor: Zoldoš, Vlatka
Date: 2015
Number of Pages: 42
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 09 Mar 2016 10:11
Last Modified: 09 Mar 2016 10:11
URI: http://digre.pmf.unizg.hr/id/eprint/4562

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