Antitumor efficacy of curcumin, bleomycin and hyperthermia on Ehrlich ascites tumor cells

Strujić, Matija (2015) Antitumor efficacy of curcumin, bleomycin and hyperthermia on Ehrlich ascites tumor cells. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Chemotherapy is one of the most advanced and successful actions in the fight against cancer. Chemotherapy is used to stop proliferation and spreading of cancer cells, as well as to initiate apoptosis in cancer cells. Due to its necrotic nature chemotherapy usually causes damager to nearby healthy tissue. Flavonoids already have shown their capability to fight cancer cells and to prevent some damage to healthy cells. In this research curcumin (1,7-bis(4-hidroksi-3-metoksifenil)hepta-1,6-dien-3,5-dion) was tested in vitro for its effect on Ehrlich ascites tumor (EAT) cells, their proliferation and the ability to initiate apoptosis/necrosis in the cells, and curcumins effect in interaction with bleomycin and hyperthermia. EAT was grown in Swiss albino mice, the cells were isolated on the 7th day, they were incubated in the culture medium, and treated with curcumin, bleomycin and hyperthermia. The antiproliferative effect of the test components was confirmed by a colorimetry assay (MTT assay) after a short incubation directly with bleomycin for 2 h and curcumin next 2 h at physiological (37 °C) or hyperthermal (43 °C) conditions and after the treatment of cells for 24 hrs. I analyzed the percentage of tumor cells in apoptosis and necrosis in flow cytometry and studied genotoxic effects using comet assay. Curcumin demonstrated the potential to inhibit the proliferation of EAT cells in vitro, and to slightly increase the percentage of cells in apoptosis. The combination with cytostatic bleomycin and hyperthermia provides a better anti-proliferative effect which is based on the high percentage induction of necrosis and complete cell lysis.

Item Type: Thesis (Diploma thesis)
Keywords: Ehrlich ascites tumor, bleomycin, curcumin, hyperthermia
Supervisor: Oršolić, Nada
Date: 2015
Number of Pages: 42
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 10 Mar 2016 09:52
Last Modified: 10 Mar 2016 09:52
URI: http://digre.pmf.unizg.hr/id/eprint/4568

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