Variability of major histocompatibility complex class II genes in the golden jackals (Canis aureus) from Israel

Mešin, Mario (2015) Variability of major histocompatibility complex class II genes in the golden jackals (Canis aureus) from Israel. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The genes of major histocompatibility (MHC) system are a key component of the immune system in mammals and are important molecular markers of adaptive genetic diversity in wild animal populations. Greater diversity of MHC genes within the population increases the chances of defence against pathogens and parasites, and thus the prospects for long-term survival. Golden jackal (Canis aureus) belongs to the family Canidae (dogs), suborder Caniformia, order Carnivora (carnivores). So far there are no published studies of MHC gene diversity on this species. The aim of this graduate thesis is to determine the level of diversity of the MHC system class II DQA, DQB and DRB genes in golden jackals from Israel. Three alleles at DQA locus, five alleles at DQB locus and four alleles at the DRB locus were found in 30 samples of jackal's ear. Methods used were DNA isolation, PCR method, electrophoresis, sequencing and molecular cloning. We found one new unique allele on DRB locus (DRB1*04802var). The rest of the alleles found are known from previous research on the other species of the dog family, which confirms the existance of a trans-species polymorphism. DQB and DRB loci show great diversity of alleles, large evolutionary distances, high ratio of non-synonymous versus synonymous substitutions, confirming the assumption that loci are influenced by positive selection.

Item Type: Thesis (Diploma thesis)
Keywords: MHC, Canis aureus, DQA, DQB, DRB
Supervisor: Galov, Ana
Co-supervisor: Arbanasić, Haidi
Date: 2015
Number of Pages: 45
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 10 Mar 2016 09:59
Last Modified: 10 Mar 2016 09:59
URI: http://digre.pmf.unizg.hr/id/eprint/4569

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