Quantification of antibiotics in honey by liquid chromatography tandem mass spectrometry

Budimir, Jelena (2016) Quantification of antibiotics in honey by liquid chromatography tandem mass spectrometry. Diploma thesis, Faculty of Science > Department of Chemistry.

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Abstract

Quantitative and qualitative method was used for determination of sulfonamides and chloramphenicol in honey by liquid chromatography coupled to mass spectrometry. Conditions were optimized for simultaneous extraction of sulfonamides and chloramphenicol from honey in solid state. Lower detection limit was achieved for sulfonamides (5.0 μg/kg). Detection limit for chloramphenicol was 0.3 μg/kg. Samples were concentrated and refined by sold phase extraction. Further analysis was conducted by data of analyte retention at the C18 column chromatography and by the MS/MS data obtained with ABSciex Qtrap 4500 system. pH value of sample and the volume of elution solvent were optimized. Repeatability of method was determined and expressed as relative standard deviation (RSD). Extraction recoveries were high with values in the range 84.62 % for chloramphenicol and of 80.80 % to 125.00 % for sulfonamides. Isotopically marked chloramphenicol was used as internal standard for quantitative analysis of antibiotics. Method was applied for determination of antibiotic in honey samples obtained from the various geographical locations.

Item Type: Thesis (Diploma thesis)
Keywords: antibiotics, chloramphenicol, honey, liquid chromatography coupled to mass spectrometry (LC-MS/MS), sulfonamides
Supervisor: Galić, Nives
Date: 2016
Number of Pages: 59
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Depositing User: Branka Maravic
Date Deposited: 10 Mar 2016 13:02
Last Modified: 10 Mar 2016 13:02
URI: http://digre.pmf.unizg.hr/id/eprint/4581

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