Expression of proteins sFRP3 and DVL3, key molecules of wnt signacpathway in glioblastoma

Gabud, Tea (2015) Expression of proteins sFRP3 and DVL3, key molecules of wnt signacpathway in glioblastoma. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Glioblastoma patients have a median survival of less than a year. Thesis aim is to identify key proteins' (sFRP3 and DVL3) status in Wnt signaling pathway in human glioblastoma samples, search for their connection with clinical data and point out possible new target therapy objects. Proteins are detected by immunohistochemistry. Tissues are analyzed under a light microscope (Olympus BH-2). The expression levels are determined by the semiquantitative method in the 3-stage signal strength with tissue and subcellular localization labels. Both proteins are expressed in glioblastoma. There is no correlation between their expression, as well as signal strength correlation with clinical parameters. Strong signal dominance was brought into connection with simultaneous localization of DVL3 in the cytoplasm and nucleus, and sFRP3 simultaneously in the cytoplasm and extracellularly along with the absence of signal in the nucleus. Prognosis for glioblastoma patients are bad even with the combination of all available therapies. It is one of the most aggressive and malignant tumor types, often inoperable due to localization. Glioblastoma is highly heterogeneous in origin and histopathological images so developing methods for detecting individual condition traits are essential. Only a more detailed knowledge of signaling pathways network could give the optimal therapeutic solutions.

Item Type: Thesis (Diploma thesis)
Keywords: immunohistochemistry, cancer, malignant glioma, β-catenin, signaling, DAB
Supervisor: Pećina Šlaus, Nives
Co-supervisor: Lacković Venturin, Gordana
Date: 2015
Number of Pages: 47
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 23 Mar 2016 11:06
Last Modified: 23 Mar 2016 11:06
URI: http://digre.pmf.unizg.hr/id/eprint/4600

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