Recombination frequency between genes of the human major histocompatibility complex

Mulanović, Marija (2015) Recombination frequency between genes of the human major histocompatibility complex. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

In this study we analyzed the recombination frequency between loci HLA-A and -B, and between loci HLA-B and -DRB1 in the group of 2049 families from the registry of hematopoietic stem cells recipients. All subjects were tested by one of the methods: microlymphocytotoxicity test or by polymerase chain reaction and primers specific for a particular allele or group of alleles (methods: PCR-SSP and PCR-SSO). The presence of crossingovers has been determined in 3.61% of families, out of which in 3.47% of families the crossingover has been determined in one child, and in 0.15% families the presence of crossingovers revealed in two children. The recombination frequency between loci HLA-A and -B was 0.61%, while between loci HLA-B and -DRB1 was 0.77%. Analysis of crossingover origin in the family revealed that the recombination frequency in oogenesis was 1.8 times higher than the recombination frequency in spermatogenesis. Among haplotypes with crossingovers between loci HLA-A and -B the crossingover was most common in those carrying the antigen/gene HLA-B27. In families with crossingovers between loci HLA-B and -DRB1 the recombination usually occurred in haplotypes positive for antigen/gene HLA-DR13. Results of this study are in concordance with the results from similar investigations.

Item Type: Thesis (Diploma thesis)
Keywords: HLA genes, recombination, crossingover
Supervisor: Grubić, Zorana
Co-supervisor: Galov, Ana
Date: 2015
Number of Pages: 49
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 23 Mar 2016 11:36
Last Modified: 23 Mar 2016 11:36
URI: http://digre.pmf.unizg.hr/id/eprint/4604

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