Estrogenic or anti-estrogenic effects of traditionally used tropic plant extracts on human U2OS cells

Zaplatić, Elizabeta (2016) Estrogenic or anti-estrogenic effects of traditionally used tropic plant extracts on human U2OS cells. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Phytoestrogens are plant-derived compounds that, due to their structural similarity with 17β- estradiol, have an ability to bind to estrogen receptors and act as their agonists or/and antagonists. They have been reported to confer health benefits related to cardiovascular diseases, hormonedependent cancers, osteoporosis, and menopausal symptoms. A tropical African plant, Mondia whitei (Hook.f.) Skeels, is used as a traditional remedy to treat various conditions and diseases, but its use is most commonly reported in the terms of male sexual problems. This indicates potential hormonal-related mechanism of action, so this study evaluated estrogenic and anti-estrogenic properties of methanol extract of Mondia whitei and its fractions. Herbal extract and fractions were tested using estrogen receptor subtype specific transactivation assays in U2OS-ERα and U2OS-ERβ cells. In the examined methanol extract, neither estrogenic nor anti-estrogenic properties have been observed. One fraction of the extract showed agonistic activity in both cell lines, whereas none of the fractions showed anti-estrogenic potential. In addition to that, applied herbal treatments were examined for cytotoxicity, but due to some confounding effects observed, MTT assay is advised to be repeated. This study was conducted as part of the bigger project, “Angola Project”, which aims to a better, science based understanding of traditionally known medicinal uses of plants.

Item Type: Thesis (Diploma thesis)
Keywords: traditional African medicine, Mondia whitei, phytoestrogens, estrogen receptor α, estrogen receptor β, human U2OS osteosarcoma cell line
Supervisor: Vollmer, Günter
Co-supervisor: Marijanović, Inga
Date: 2016
Number of Pages: 44
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 24 Mar 2016 11:25
Last Modified: 24 Mar 2016 11:26
URI: http://digre.pmf.unizg.hr/id/eprint/4611

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