The effect of perinatal exposure to increased serotonin concentrations on histological structure of somatosensory cortex of adult rats

Šoltić, Darija (2016) The effect of perinatal exposure to increased serotonin concentrations on histological structure of somatosensory cortex of adult rats. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Serotonin (5HT) is biologically active amine present in the brain and peripheral tissues of mammals where it has various functions, depending on the compartment. In the brain, during neurogenesis, it regulates the development of serotonergic neurons and target tissues, while later it assumes the function of a neurotransmitter. The aim of this graduation thesis was to examine the effect of perinatally induced high serotonin levels on the development of barrel field in the rat somatosensory cortex, induced by treatment with the immediate serotonin precursor ,5-hydroxytryptophan (5HTP, 25 mg/kg), and inhibitor of serotonin degradation, tranylcypromine (TCP, 2 mg/kg). Brains were collected on PND 21 or PND 70. Tangential sections of somatosensory cortex where used for histological processing with Nissl staining method, and histochemical method for cytochrome oxidase staining (CO). Qualitative analysis of barrel field were conducted by examination of Nissl sections under the microscope. We found barrel field on CO sections under the microscope and photographed them. In software ImageJ we draw barrels in barrel field, measured their area surface and conducted analysis of quantitative differences between treated and control group. High serotonin levels had significant effect on barrel field of the pups, with 5HTP treatment resulting in smaller barrel field and barrel area, and TCP treatment disrupting their structure. Treatment effects of 5HTP, still visible in adult rats, point to the decreased branching of thalamo-cortical axons during the prenatal phase of the treatment. Since the effects of TCP were not present in the brains of adult animals, we assume that the cronic exposure to high serotonin levels in the postnatal phase of the treatmen only delayed maturation of the barrel field.

Item Type: Thesis (Diploma thesis)
Keywords: barrel field, barrels, 5-hydroxitryptophan, tranylcypromine
Supervisor: Hranilović, Dubravka and Jovanov Milošević, Nataša
Co-supervisor: Blažević, Ana Sofia
Date: 2016
Number of Pages: 41
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 24 Mar 2016 12:31
Last Modified: 24 Mar 2016 12:31
URI: http://digre.pmf.unizg.hr/id/eprint/4618

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