Variability of major histocompatibility complex DRB class II geneand DQA/DQB/DRB haplotypes in the golden jackals (Canis aureus) from Eastern Europe

Šešelja, Kate (2016) Variability of major histocompatibility complex DRB class II geneand DQA/DQB/DRB haplotypes in the golden jackals (Canis aureus) from Eastern Europe. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The genes of major histocompatibility (MHC) system are a key component of the immune system in mammals. The diversity of MHC genes enables the recognition of a wider range of pathogens, and thus increases the chances of survival. They are important molecular markers for studying adaptive genetic diversity in populations and species. Golden jackal (Canis aureus) belongs to the family Canidae (dogs), suborder Caniformia, order Carnivora (carnivores). So far there are only a few published genetic studies concerning golden jackal although it is a widespread species. The aim of this graduate thesis is to determine the diversity of the MHC DRB class II gene in golden jackals from Eastern Europe. Using the methods of DNA isolation, PCR method, electrophoresis, sequencing and molecular cloning, I have identified five alleles at DRB locus from 21 samples. All five alleles has unique amino acid sequence, and two alleles have been newly found and named DRB*14aEst and DRB*15aEst. The allele DRB*00901 is known from previous research of other species in the dog family, which confirms the existence of a trans-species polymorphism. The dN/dS (ratio of non-synonymous versus synonymous substitutions) test has confirmed the assumption that DRB loci are influenced by positive selection. In the examined sample, total of seven unique haplotypes have been identified. For inference of haplotypes I used alleles found at DQA and DQB loci of the same samples identified in previous research.

Item Type: Thesis (Diploma thesis)
Keywords: major histocompatibility complex, Canis aureus, balancing selection haplotypes
Supervisor: Galov, Ana
Co-supervisor: Arbanasić, Haidi
Date: 2016
Number of Pages: 40
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 24 Mar 2016 12:41
Last Modified: 24 Mar 2016 12:41
URI: http://digre.pmf.unizg.hr/id/eprint/4620

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