The influence of emrBAR efflux system on symbiosis between Medicago truncatula and Sinorhizobium meliloti

Mitić, Damjan (2016) The influence of emrBAR efflux system on symbiosis between Medicago truncatula and Sinorhizobium meliloti. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Nitrogen is an essential part of biomolecules and is needed for the metabolism and development of all plants. Symbiotic interactions between bacteria and plants have evolved in order to enable the absorption of nitrogen to plants. The main focus of this study was the effect of the EmrBAR efflux system on the symbiosis between the legume Medicago truncatula and the rhizobium Sinorhizobium meliloti. The results showed that emrA and emrR are expressed in early symbiotic interactions, during the formation of infection threads. EmrA is expressed in the infection and nitrogen-fixation zones, and emrR only in the infection zone of the nodule. Analysis of nodulation phenotype of the emrBAR deletion mutants and the wild type in sloped agar tubes might suggest that the creation of new nodules was a response to inefficient nitrogen fixation. Analysis of nodulation phenotype of the emrBAR deletion mutants and the wild type in Magenta boxes suggests that the emrBAR deletion affects symbiotic plant growth by decreasing nodule efficiency. A bacterial growth assay showed that 2-phenylphenol inhibits the growth of the two emrBAR deletion mutant strains in comparison with the wild type. This seems to indicate the importance of the EmrBAR efflux system in the extrusion of toxic substances.

Item Type: Thesis (Diploma thesis)
Keywords: Medicago truncatula, Sinorhizobium meliloti, emrBAR, symbiosis, efflux system
Supervisor: Göttfert, Michael
Co-supervisor: Ivančić Baće, Ivana
Date: 2016
Number of Pages: 50
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 24 Mar 2016 15:48
Last Modified: 24 Mar 2016 15:48
URI: http://digre.pmf.unizg.hr/id/eprint/4628

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