The role of mitochondrial protein Nix dimerisation in mitophagy activation

Marinković, Mija (2013) The role of mitochondrial protein Nix dimerisation in mitophagy activation. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Recent evidence indicates that autophagy mediates selective removal of protein aggregates, organelles such as mitochondria or ribosomes and microbes in cells. Recently, the role of mitochondrial protein Nix as a receptor for selective removal of mitochondria by autophagy was described. The aim of this research was to determine the role of Nix dimerisation in mitophagy activation. Using site directed mutagenesis, two types of mutants were made, with ability to form only monomers or to form both monomers and dimers. GST Pull-down and Western blot analysis were used to determine binding of different Nix forms to autophagosomal proteins. Immunofluorescence microscopy was applied to define Nix localization in cells and to quantify mitophagic activity in normal conditions and in the presence of mitochondrial poison CCCP. As expected, results showed increased mitophagy activity in the cells overexpressing wild type Nix that form dimers. Nix mutants without the ability to form dimers showed reduced mitophagic activity. In conclusion, Nix dimerisation is a possible additional mechanism how autophagic machinery is recruited to mitoichondria to enhance their removal.

Item Type: Thesis (Diploma thesis)
Keywords: autophagy, mitophagy, mitochondria, dimerization, Nix
Supervisor: Novak, Ivana
Co-supervisor: Marijanović, Inga
Date: 2013
Number of Pages: 78
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 20 Feb 2014 14:10
Last Modified: 22 Sep 2014 12:19
URI: http://digre.pmf.unizg.hr/id/eprint/463

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