Effect of cytokine-microenvironment on dendritic cell activation

Majstorović, Ozren (2016) Effect of cytokine-microenvironment on dendritic cell activation. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Dendritic cells (DC) are a heterogeneous group of potent, professional antigen-presenting cells (APC) with characteristic morphology and unique ability to induce a primary immune response. DCs capture and transfer information from the outside world to the cells of the adaptive immune system, they are important for the induction of tolerance and the regulation of the type of T cell mediated immune response. DCs produce cytokines and are susceptible to cytokine-mediated activation. The direction of the immune response depends on a complex interaction of immune cells and a number of other factors, where microenvironment in which the cells are located plays an important role. The general objective was to investigate the influence of different cytokines on DCs activation. The specific objectives were: to generate DC from porcine blood, to evaluate the effect of different concentrations of cytokine IFNγ and IL-4 on DC activation and to analyze IL-1β, IL-10, IL-12, IL-23 i TGFβ gene expression, as indicators of type of immune response that was induced trough specific cytokine. The results have shown that proinflammatory cytokine IFNγ activated DCs by causing increased expression of proinflammatory cytokines IL-1β, IL-12, IL-23 and inhibiting the expression of anti-inflammatory cytokines IL-10 and TGFβ, whereas treatment with anti-inflammatory cytokine IL-4 showed the opposite effect. It was also demonstrated that cytokine concentration has a distinct role in regulating expression levels trough dose-dependent mechanism.

Item Type: Thesis (Diploma thesis)
Keywords: monocyte-derived dendritic cells (MoDC), Th response, qRT-PCR, pigs
Supervisor: Faldyna, Martin
Co-supervisor: Korać, Petra
Date: 2016
Number of Pages: 43
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 24 Mar 2016 16:06
Last Modified: 24 Mar 2016 16:06
URI: http://digre.pmf.unizg.hr/id/eprint/4631

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