Intermethod comparison of detection performance for allergen-specific IgE antibodies between ImmunoCAP and IMMULITE and their agreement with skin prick testing

Knezović, Anela (2016) Intermethod comparison of detection performance for allergen-specific IgE antibodies between ImmunoCAP and IMMULITE and their agreement with skin prick testing. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Prevalence of allergic diseases has been in dramatic increase over last 20-30 years, especially in children. The reason of this increasing trend is still unknown. It's known that allergic disease has several phases: sensibilisation, symptoms manifestation, persistence and remision. Modern medicine tends to prevent, to preclude progression of the disease and to diagnose disease in the earliest phases. A key part of diagnosing allergic diseases is detection of allergen that causes reaction. Today, diagnostics of allergic diseases is carried out by in vivo and in vitro tests. General in vivo test is a skin prick test that measures skin reactions at specific allergen injected into the skin. General in vitro tests measures the level of specific IgE in a sera sample. The goal of this research was to compare the results of measurements of specific IgE antibodies measured by ImmunoCAP and IMMULITE methods, and to compare that results with the results of skin prick test on the same allergens. We compared mentioned methods processing selected and nonselected group of samples. The results showed that IMMULITE and ImmunoCAP are comparable and approximately equally sensitive methods. The IMMULITE showed better correlation with the skin prick test results. Both methods are valid in the routine diagnostics of allergic diseases, but no method is sufficient nor ideal in diagnostics of allergic diseases.

Item Type: Thesis (Diploma thesis)
Keywords: allergic diseases, specific IgE, in vivo, in vitro, ImmunoCAP, IMMULITE
Supervisor: Turkalj, Mirjana
Co-supervisor: Oršolić, Nada
Date: 2016
Number of Pages: 39
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 24 Mar 2016 23:38
Last Modified: 24 Mar 2016 23:38
URI: http://digre.pmf.unizg.hr/id/eprint/4638

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