Analysis of the quorum sensing role in the Rhizobium-Leguminosae symbiosis

Busch, Franka (2016) Analysis of the quorum sensing role in the Rhizobium-Leguminosae symbiosis. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

In this study the role of quorum sensing communication in Rhizobium-Leguminosae symbiosis was examined in the process of nitrogen fixation. In order to understand better the quorum sensing communication with the methods of molecular engineering mutants in cinR and traI genes, which are the part of the bacterial strains genome of Rhizobium leguminosarum bv. viciae UPM791, were constructed. Genes cinR and traI are chosen because they control the AHL-dependent quorum sensing system. Mutants of genes cinI and traI are successfully obtained and further characterized at the laboratory for phenotypes in interactions with plants. Preliminary results obtained by TLC analysis showed that the cinR mutant is producing less C14-HSL signals, also showing a reduction on the level of short-chain AHLs. Analysis of traI gene showed no significant alteration on C14-HSL production but the level of short-chain AHLs is reduced. Preliminary results have to be confirmed in further analysis to assess better examination of the significance of mutations.

Item Type: Thesis (Diploma thesis)
Keywords: bacteria, signal molecules, homoserin lactone, aycl-homoserin lactone, nodulation
Supervisor: Hrenović, Jasna
Date: 2016
Number of Pages: 54
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 25 Mar 2016 09:38
Last Modified: 25 Mar 2016 09:38
URI: http://digre.pmf.unizg.hr/id/eprint/4655

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