The use of inteins in protein splicing

Gabrilo, Jelena (2015) The use of inteins in protein splicing. Bachelor's thesis, Faculty of Science > Department of Biology.

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Abstract

Protein splicing is a post-translational process by which an intervening sequence, called an intein, catalyses its own excision from the polypeptide as well as ligation of the flanking sequences, called exteins. Some inteins are expressed as two separate fragments and facilitate protein trans splicing. Some of them can undergo conditional protein splicing whereas expressed protein ligation (EPL method) can incorporate site – specific protein modifications. Intein – based methods have become an essential tool in modern biotechnology. Progress in understanding the structure and catalytic properties of cis- and trans- inteins has led to the development of modified inteins that allow for protein purification, ligation and cyclization. They have been used in protein expression and modification, post – translational processing and labelling and protein regulation.

Item Type: Thesis (Bachelor's thesis)
Supervisor: Ivančić Baće, Ivana
Date: 2015
Number of Pages: 19
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 13 Apr 2016 12:39
Last Modified: 13 Apr 2016 12:39
URI: http://digre.pmf.unizg.hr/id/eprint/4707

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