Bridging microtubules and generation of forces in mitosis

Risteski, Patrik (2015) Bridging microtubules and generation of forces in mitosis. Bachelor's thesis, Faculty of Science > Department of Biology.

Language: Croatian

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At the onset of division, cell constructs a spindle, complex cytoskeletal mechanism formed with microtubules and microtubule-associated proteins. The current model of the mitotic spindle defines two separate populations of microtubules; kinetochore and nonkinetochore microtubules. During metaphase, forces on the kinetochores are exerted by kinetochore fibers consisting of kinetochore microtubules. However, non-kinetochore microtubules have been observed in the vicinity of the kinetochores in metaphase. This paper summarizes all research based on subpopulation of antiparallel microtubules, named bridging microtubules that span the region under sister kinetochores and facilitate a lateral connection between corresponding kinetochore fibers. Bridging microtubules have been visualized by confocal microscopy in HeLa and PtK1 cells. In order to distinct bridging microtubules from other non-kinetochore microtubules and to test forces that bridging bundle generates, laser ablation assay was developed. Physical model in which bridging bundle provides mechanical resistance to the tension and compression within the kinetochore fibers was constructed. Results indicate that the new subpopulation of microtubules is responsible for precise chromosome segregation and organization by balancing forces within mitotic spindle.

Item Type: Thesis (Bachelor's thesis)
Supervisor: Balen, Biljana
Date: 2015
Number of Pages: 26
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 14 Apr 2016 13:40
Last Modified: 14 Apr 2016 13:40

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