The role of hair in forensic analysis

Skube, Karlo (2015) The role of hair in forensic analysis. Bachelor's thesis, Faculty of Science > Department of Biology.

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Abstract

Hair is one of the most common evidence used in forensic analysis. Due to a simple transfer, it can easily connect a suspect to a crime scene or victim. Comparison microscope is used in microscopic comparison of known and unknown hair sample. Microscopic analysis of the hair can help determine someone’s race, part of the body where hair was growing, age and gender. If it has preserved root or parts of surrounding follicular tissue, it can be used for nuclear DNA isolation, profiling and individualization of a person. Mitochondrial DNA can also help in identification but it cannot individualize a person because it is inherited maternally. Improvement in existing methods with simple advancements such as fixation of hair in absolute ethanol and development of new methods significantly increases efficiency of DNA isolation. Using different colors such as Harris’s Haematoxylin, improvement occurs in two things. The first is to reduce security risks because Harris’s Haematoxylin is much more safer than DAPI. Second improvement is the elimination of the need to use fluorescent microscope because DNA is visible without simulating fluorescence by certain wavelengths.

Item Type: Thesis (Bachelor's thesis)
Supervisor: Marijanović, Inga
Date: 2015
Number of Pages: 19
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Silvana Šehić
Date Deposited: 14 Apr 2016 13:56
Last Modified: 14 Apr 2016 13:56
URI: http://digre.pmf.unizg.hr/id/eprint/4745

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