Cadmium- and copper- induced genotoxicity by random amplified polimorphic dna in tobacco

Drempetić, Ana (2012) Cadmium- and copper- induced genotoxicity by random amplified polimorphic dna in tobacco. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Heavy metals are persistent environmental pollutants, and can have toxic effects due to their accumulation in living organisms. Effect of heavy metals at different levels of biological organization is generally well known, but the combined effect of several heavy metals on the organism has been insufficiently studied. The genotoxic effect of cadmium and copper, alone and in combination, in tobacco Nicotiana tabacum L., was investigate in this study. The aim was to determine the polymorphism of DNA as a result of tobacco exposure to heavy metals cadmium and copper, and evaluate if the combined effect of these metals is synergistic or antagonistic. Metal content analysis revealed that the influence of copper and cadmium depended on the concentration and developmental stage of plants. Copper increased toxic effects of cadmium in the seedlings (synergistic effect), whereas in adult plants reduce toxic effects of cadmium (antagonistic effect). Seedlings have reduced genome stability in comparison to adult plants.

Item Type: Thesis (Diploma thesis)
Keywords: Nicotiana tabacum L., copper, zinc, cadmium, genotoxicity, random amplified polimorphic DNA, bioacumulation
Supervisor: Pavlica, Mirjana
Date: 2012
Number of Pages: 47
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 25 Feb 2014 12:26
Last Modified: 19 Sep 2014 09:47
URI: http://digre.pmf.unizg.hr/id/eprint/492

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