Phenolic compounds in strawberry tree (Arbutus unedo L.) and their biological effects

Jurica, Karlo (2016) Phenolic compounds in strawberry tree (Arbutus unedo L.) and their biological effects. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

This thesis investigated the phytochemical characteristics, antimicrobial properties and biological effects of strawberry tree leaves. Total phenol, tannin, flavonoid and phenolic acid content was determined as well as polyphenolic profile using the UHPLC-MS/MS Orbitrap method. Arbutin and hydroquinone concentrations were measured by HPLC-UV/DAD, antioxidative properties by FRAP, TEAC, DPPH, MIC concentrations by a Twofolded Microdilution method and mutagenicity by Comet-test and CBMN test. Thirty-seven new phenolic compounds were detected. Leaves contained 0.3% of arbutin while hydroquinone was not detected. Methanol was shown to be a more effective solvent for the extraction of polyphenols. Methanolic extract showed better antioxidative properties and better antimicrobial effect on standard ATCC and clinical microorganism strains. Arbutin did not show antimicrobial activity, while its metabolite hydroquinone showed positive antimicrobial activity against the tested uropathogens. Water extract applied at an arbutin concentration corresponding to the maximum allowable daily intake showed low genotoxicity in vitro and in vivo. Strawberry tree water leaf extract has great potential as a phytotherapeutic in clinical application.

Item Type: Thesis (Doctoral thesis)
Keywords: Strawberry tree leaves, polyphenolic profile, arbutin, hydroquinone, antioxidative properties, antimicrobial properties, mutagenicity
Supervisor: Dario, Kremer and Vesna, Benković
Date: 2016
Number of Pages: 161
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 05 Jul 2016 09:10
Last Modified: 05 Jul 2016 13:34
URI: http://digre.pmf.unizg.hr/id/eprint/4944

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