Association of FasL and FOXO3 genes polymorphisms frequency with levels of TNFα and IL-10 cytokines in rheumatoid arthritis

Artuković, Marinko (2016) Association of FasL and FOXO3 genes polymorphisms frequency with levels of TNFα and IL-10 cytokines in rheumatoid arthritis. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

FOXO3 and FasL single nucleotide polymorphisms (SNP) could be related to the course of rheumatoid arthritis (RA) due to changed expression of certain cytokines. FasL gene SNP increases the susceptibility to RA. Both genes are involved in apoptosis, with FOXO3 transcription factor suppression of Fas ligand preventing apoptosis and sustaining chronic inflammation. We determined the frequency of SNPs, expression of proinflammatory (TNFα) and anti-inflammatory (IL-10) cytokines in RA patients by methods of DNA isolation from peripheral blood, genotyping, determination of TNFα and IL-10 (ELISA) and than correlated polymorphisms with expression of cytokines. Results: In our cohort FOXO3 and FasL polymorphisms does not differ in frequency in RA, and are not related to expression changes of TNFα and IL-10 with consequences to the course of RA.

Item Type: Thesis (Doctoral thesis)
Keywords: RA, SNP, FOXO3 and FasL genes, cytokines TNFα and IL-10
Supervisor: Stipić Marković, Asja
Co-supervisor: Kelava, Tomislav
Date: 2016
Number of Pages: 86
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 05 Jul 2016 10:34
Last Modified: 05 Jul 2016 10:34
URI: http://digre.pmf.unizg.hr/id/eprint/4945

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