Effect of sex hormones on the expression of chloride/formate exchanger (Cfex, Slc26a6) in rat organs

Karaica, Dean (2016) Effect of sex hormones on the expression of chloride/formate exchanger (Cfex, Slc26a6) in rat organs. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

The chloride/formate exchanger (Cfex/Slc26a6) is a transmembrane protein mediating chloride, bicarbonate, oxalate, formate and hydroxyl ions transport in mammals. Previous findings support its possible role in the oxalate urinary tract stones formation, with higher incidence in men. Due to lack of data in rats, as commonly used experimental animals, its cell localization and possible sex-related expression was investigated in various rat organs/tissues. Specificity of anti-CFEX antibody was verified by heterologous expression systems using a full-length rCfex cDNA-transfected HEK293 cells. By RT-PCR, rCfex mRNA was detected in the kidneys, liver, pancreas and small intestine. By immunofluorescence, protein rCfex was localized in the apical membrane of enterocytes and renal proximal tubules, hepatocyte canalicular membranes, and luminal domain of pancreatic ducts. Its renal expression was downregulated by castration, and upregulated by testosteron. Therefore, the rCfex mRNA/protein is expressed in various rat organs/tissues, whereas its renal expression is maledominant due to post-pubertal androgen stimulation.

Item Type: Thesis (Doctoral thesis)
Keywords: membrane transporters, immunolocalization, sex differences, intestine, kidney, liver, pancreas
Supervisor: Breljak, Davorka
Date: 2016
Number of Pages: 153
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 05 Jul 2016 11:07
Last Modified: 05 Jul 2016 11:07
URI: http://digre.pmf.unizg.hr/id/eprint/4947

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