Resistance of the proteomes of immortal cell lines to oxidative stress

Nikolić, Andrea (2016) Resistance of the proteomes of immortal cell lines to oxidative stress. Doctoral thesis, Faculty of Science > Department of Biology.

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The excessive production of reactive oxygen species (ROS) in cells induces irreversible protein modifications, carbonylation, that impairs protein structure and function, as well as proteome stability. The correlation between proteome damage and cell death is well established in bacteria and invertebrates and the aim of this research was to investigate the relationship between mortality and protein damage of immortal cell lines derived from primary tumor adenocarcinoma SW480 and its metastasis SW620. We found that SW620 cell line is more resistant to UVC-induced oxidative damage relative to SW480 cells. The reason for greater resistance of SW620 cells could be lower ROS level compared to SW480 and greater proteome stability. The differential analysis of proteomes of two cell lines after 90% UVC-induced mortality shows that SW480 displays greater sensitivity due to the damage of proteome quality system and the ability to remove oxidative damaged proteins. Also, we identified proteins that could be possible targets in anti-cancer therapy.

Item Type: Thesis (Doctoral thesis)
Keywords: protein oxidation, cell death, reactive oxygen species, primary tumor, metastasis
Supervisor: Kralj, Marijeta
Date: 2016
Number of Pages: 119
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 05 Jul 2016 13:06
Last Modified: 05 Jul 2016 13:06

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