Biomonitoring of Vrana Lake by cage exposure of mussel Unio pictorum (Linnaeus 1758)

Basrek, Luka (2012) Biomonitoring of Vrana Lake by cage exposure of mussel Unio pictorum (Linnaeus 1758). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Pollution of aquatic ecosystems can cause changes on molecular and cellular level in organisms inhabiting them They usually occur before toxic action becomes apparent in physiology of the entire organism. In this study, oxydative damage of macromolecules and DNA damage were examined using caged painter's mussels (Unio pictorum L.) as test organism. Mussels were cage exposed at several locations on Vrana lake (Channel Kotarka, Lateral channel, Lake centre, Prosika). Mussels were collected at the reference site (Draganići), and exposed during spring and autumn 2011. Biomarkers of genotoxicity (Comet assay and micronucleus-test) and biochemical indicators of stress (total proteins, malondialdehyde, carbonyl groups) were analysed on mussels exposed in Vrana Lake and the ones from the reference site. DNA damage and oxidative damage of macromolecules were recorded on Channel Kotarka and Lateral channel which indicates presence of genotoxicants as well asagents which cause oxidative damage in Vrana Lake.

Item Type: Thesis (Diploma thesis)
Keywords: Unio pictorum, haemocytes, comet-test, micronucleus-test, malondialdehyde, carbonyl groups, oxydative damage, environmental contamination
Supervisor: Klobučar, Goran
Date: 2012
Number of Pages: 48
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 25 Feb 2014 12:53
Last Modified: 18 Sep 2014 12:38
URI: http://digre.pmf.unizg.hr/id/eprint/497

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