NO-system and Pentadecapeptide BPC 157 in the Esophagogastric Anastomosis Healing Process in Rat Model

Djaković, Željko (2016) NO-system and Pentadecapeptide BPC 157 in the Esophagogastric Anastomosis Healing Process in Rat Model. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Esophagogastric anastomosis in human surgery is part of the surgical treatment of esophageal cancer. Anastomotic leak is responsible for a third of all perioperative deaths. We suggest an enhanced healing of esophagogastric anastomosis, NO-system dependent, with stable gastric pentadecapeptide BPC 157. Throughout 4 days after esophagogastric anastomosis creation, rats received medication (/kg ip once daily: BPC 157 (10pg, 10ng), L-NAME (5mg), L-arginine (100mg) alone and/or combined). Daily assessment includes damage in stomach (sum of longest diameters, mm), esophagus (esophagitis, scored 0-4), anastomosis (ml H2O before leak), pressure in pyloric sphincter and in esophagus at anastomosis (cmH2O) and weight loss (g). In the control group we noted progressing stomach damage, severe esophagitis, rapid anastomosis leak, decrease of pressure, severe in pyloric sphincter as well as less pressure assessed in esophagus at anastomosis, alongside with prominent weight loss. By contrast, BPC 157 treated rats have almost no gastric lesions or esophagitis; anastomosis sustains maximal water volume without leakage; higher pressure in pyloric sphincter, values in esophagus at anastomosis close to normal values in lower esophageal sphincter, alongside with markedly less weight loss. In Larginine rats we noted a beneficial effect comparable to that of BPC 157 regimens. By contrast to BPC 157 and L-arginine beneficial effects, L-NAME rats exhibited all parameters of esophagogastric anastomosis markedly aggravated. L-NAME+L-arginine-rats presented control values; L-arginine+BPC 157 rats presented a beneficial effect but no augmentation of the previous separate effects. BPC 157 completely counteracted L-NAME effects, and maintained its original beneficial effect in L-NAME+BPC 157 and L-NAME+L-arginine+BPC 157 combination. Failed esophagogastric anastomosis healing appears as a NO-system disturbance, and BPC 157 in interaction with the NO-system markedly improves the healing of esophagogastric anastomosis.

Item Type: Thesis (Doctoral thesis)
Keywords: esophagogastric anastomosis, NO-system, pentadecapeptide BPC 157, healing proces, pyloric sphincter
Supervisor: Sikirić, Predrag and Stančić-Rokotov, Dinko
Date: 2016
Number of Pages: 76
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 15 Jul 2016 09:51
Last Modified: 15 Jul 2016 09:51
URI: http://digre.pmf.unizg.hr/id/eprint/4979

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